FastQCFastQC Report
Mon 20 Mar 2023
SRR6502987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62328356
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2018755832.389042958232366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA879505914.110847075767568No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4908570.7875340077957454No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT4430960.7109059638922612No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4261230.6836743776781149No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4099430.6577150855703622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAG3802400.6100594085940595No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3747650.6012752847195264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG3528720.5661500200647038No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3114980.49976931847841455No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3035280.4869822011669937No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2771710.44469486729282576No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2674220.4290535113745019Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2594340.4162375147517127No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2578890.4137587071925979No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG2441980.3917927820846101No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA2412120.3870020252098419No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2407670.3862880644565693No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2264130.3632584180465148No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2227010.3573028622798907No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2226700.3572531256880897No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2226610.35723868603240555No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2201590.35322446175220795No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2197490.3525666552154849No Hit
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC2178750.3495599980208045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG2177470.349354634028852No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA2123070.34062666437086836No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2119420.34004105611256613No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA2106330.33794088841361386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA1878740.3014262080007373No Hit
CAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG1784590.28632072374891454No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG1698310.2724779071663626No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1669840.2679101627516054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAAAGCAGTGGTATCAACGCAGA1657380.26591107264244224No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1582690.25392776283077323No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1558220.2500017808908677No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT1547930.24835084692431164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCGG1534640.24621859110161676No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1456490.23368015674920095No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGG1445960.23199071703415378No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1421250.22802622934575714No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1420080.22783851382186304No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1406660.2256854007187355No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG1348340.21632850383539715No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1333080.21388017999383777No Hit
AAGCAGTGGTATCAACGCAGAGTACATGCAAGCAGTGGTATCAACGCAGA1332050.21371492615656346No Hit
AGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT1288870.20678710024053898No Hit
AAGCAGTGGTATCAACGCAGAGCACACGTCTGAACTCCAGTCACTTAGGC1250280.20059569676440686TruSeq Adapter, Index 3 (100% over 32bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA1170060.18772515033125534No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC1160990.18626995391952902No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT1102310.17685529841345407No Hit
CGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT1098640.17626648134277761No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1098190.17619428306435678No Hit
AACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA1097530.17608839225600623No Hit
AAGCAGTGGGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1077420.17286193141368913No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1069030.17151583462268763No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG1050320.16851399064656863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCCGA1030940.165404651455912No Hit
CAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC1026510.1646938995150137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCA1024310.1643409301538452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGGTGGTATCAACGCAG936130.15019327639573873No Hit
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA885640.1420926295569227No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG875340.1404400911841795No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACCGCAG841980.135087792143916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA835310.13401765321710074No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT830330.13321865893591034No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA783970.12578063185237873No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC781530.12538915674271917No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA757780.12157869204828697No Hit
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT736560.11817414211919852No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA732730.11755965454952799No Hit
TGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAT724590.11625366791320471No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC712150.11425778661641581Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC698980.11214478366796649No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA678950.1089311580751464No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG630190.10110807350670376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCAA904600.040.599739
AGTGGGA940600.038.4719775
AAGCAGT173955400.035.869511
GTATCAA183250800.033.9967279
AGTGGTA182713000.033.980795
AGCAGTG184736950.033.8011172
GGTATCA188202350.033.10378
GGGATCA1115450.032.944658
GCAGTGG193379850.032.2977753
CAGTGGT193699750.032.066984
GTGGTAT194968800.031.9503216
TGGTATC195202500.031.9246437
GTGGGAT1358300.026.9962026
CAGTGGG1469100.025.1414034
CCTAACT58900.018.7512560-64
GGAATTC108350.018.54055230-34
AAGCTGT1125750.017.4302641
AATGGAC64850.016.74452670-74
GATAAGT67450.016.65344695-99
ATTAGGG68150.016.22576345-49
Produced by FastQC (version 0.11.5)