FastQCFastQC Report
Mon 20 Mar 2023
SRR6502986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113805838
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA24181832.124832119772274No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18725031.6453488089073252No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8256650.725503203095785No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC3698300.32496575439302156No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1973130.17337687017426998No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1569660.13792438310590008No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1526520.1341337164091705No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1214070.10667906157854573Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGC4770800.0141.701632
CGTGAGA4725100.0140.954482
ATAGCGA1780800.0140.568131
CCGTGAG4769100.0140.296591
TAGCGAC1776450.0140.078172
CGCATAC2166350.0139.744251
GAGTTAG4874600.0138.969041
ACTTCGA891300.0137.468282
GTTAGCT1986550.0137.411993
GCATACA2202300.0137.129912
CCGAAGT1982800.0136.884631
CGAAGTA1988700.0136.757022
AGCGACT852650.0136.663533
GTCGTAG1210300.0136.658861
GTTAGCG1477250.0136.408313
ATGAATC1077150.0136.34872
GCTAACT993400.0136.228873
AGGCTAA2065700.0135.946091
GGCTAAC2065150.0135.494632
GTTAGCA1534200.0134.908223
Produced by FastQC (version 0.11.5)