FastQCFastQC Report
Mon 20 Mar 2023
SRR6502985_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502985_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133104643
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16111841.2104641608933204No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13770641.0345724754319803No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA5560930.41778632770909424No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA3571470.26832046722817926No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1524190.11451065610085442No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1446040.10863933574428355No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1376460.10341186971216325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGA3074650.0140.809231
GCGAGTA3998950.0140.666551
AACATCG5384400.0140.658782
ACGTGAT3112400.0140.186722
CCGAGAT3227050.0140.05333
CTAACGA1443150.0139.97162
ACGCTTA1952050.0139.931812
ACCTTAC4519250.0139.621292
CGAGTAA4002750.0139.411322
AGTGGTC4618000.0139.402731
ACCGAGA5901100.0139.313952
GATAGAC1456400.0139.042881
GTGGTCA4631300.0138.643422
AACCGAG6025200.0138.60241
ACATCGA1754050.0138.484943
CACCTTA4555500.0138.026641
TACGCAA1201450.0137.526182
ATGCCTA1367500.0137.485431
AGTTAGC1528050.0137.399462
AGAGTCA1424650.0137.121751
Produced by FastQC (version 0.11.5)