FastQCFastQC Report
Mon 20 Mar 2023
SRR6502984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84630315
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1194675014.116395525645864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15609351.8444159164479064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA8618101.0183230441715831No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3524730.4164855111315608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3280600.38763887384798223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3011150.35580040083745407No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2789200.3295745738391734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC2318570.2739644771498251No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2283150.26977921563921864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA2005620.23698600200176495No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1391630.16443634884261035No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1156900.13670042466461338No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1142530.13500245154469764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG1114390.13167740188607358No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1037230.12256010154281004No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC919070.10859820148371184No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT901580.10653156614151796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA893150.10553546917555488No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT884710.104538190599905No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA854000.10090946725177614No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC848000.10020050143970279No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA847670.10016150832003876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT83754900.063.0122451
GTATCAA87404850.060.454439
AGTGGTA87736500.060.1827665
AGCAGTG87835000.060.120752
GGTATCA89242400.059.198618
CAGTGGT90712800.058.2361764
GTGGTAT91840850.057.5105326
TGGTATC91903550.057.492377
GCAGTGG92142750.057.331763
ACACTCG550100.023.86247830-34
CGATAGG140500.022.8550535-39
CGACCTA217350.022.47900445-49
TACGCAC224450.021.83208550-54
TGGAACG609050.020.86483440-44
CTAGTCC623300.020.77132470-74
GAAACCG501200.020.74622785-89
CGCTTCT657350.020.4840135-39
ATGGGGC2607700.020.46723725-29
GGGACAC737250.020.4378825-29
CACTCGC634900.020.2738630-34
Produced by FastQC (version 0.11.5)