FastQCFastQC Report
Mon 20 Mar 2023
SRR6502983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73709117
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC15845192.14969201164084No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2940620.3989492914424683No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2152210.29198694647230683No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1806240.2450497405904347No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1421090.19279704571688194No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG783790.10633555683484852Illumina Single End PCR Primer 1 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGA2874900.0142.655852
ATGCCTA1533900.0141.299761
AGTCACT1728700.0141.163711
ACGTGAT1610800.0141.050952
GTCACTA1724100.0140.773282
AACGTGA1605750.0140.697741
CACTTCG2938250.0140.552871
CGTGAGA2490450.0140.51052
AGAGTCA1367100.0140.219311
CAAGCTA1250350.0139.511112
CCGTGAG2527200.0139.48981
CGCATAC1153200.0138.796341
AAGACTA1512650.0138.63082
CAAGACT1517150.0138.612581
TGCCTAA1549350.0138.601642
CTGGCAT1785100.0138.587361
GAGTCAA1368350.0138.572682
CTCAATG1363350.0138.403261
ACTAGTA2442450.0137.906952
CCGAAGT1066650.0137.644991
Produced by FastQC (version 0.11.5)