FastQCFastQC Report
Mon 20 Mar 2023
SRR6502983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73709117
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1773297524.058048341564042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27267513.6993402050929465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA4685360.6356554237381516No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4205830.5705983426717756No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2093070.2839635156665898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2063990.2800182777932342No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1839530.249566142543805No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1793710.2433498151931463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1721580.23356405151346477No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1711140.23214767312977036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1654460.2244579866558434No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1512740.20523105710247486No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1486910.20172674161867926No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1409220.19118666148178115No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1308320.17749771714128662No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1276680.17320516809338524No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1240840.16834281164974477No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1187130.1610560604056619No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1186400.16095702245354535No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1156730.15693174020793113No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1132020.15357937336299932No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC1091630.1480997255739748Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1067730.14485725015536408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA1058050.14354397977661298TruSeq Adapter, Index 2 (95% over 22bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1050050.14245863235615752No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1012580.13737513637559925No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG946340.128388459734228No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC930600.12625303868448187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA899150.12198626663781634No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT840010.1139628358320993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA837150.11357482412928649No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTGTCAACGCAG830260.1126400686634192No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG742200.1006931069327557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT112505150.050.694411
AGCAGTG117182900.048.6935542
AGTGGTA117536300.048.546415
GTATCAA118112000.048.3426789
GGTATCA120484600.047.380668
CAGTGGT122169150.046.7218974
GCAGTGG122861050.046.4576073
TGGTATC123404050.046.263777
GTGGTAT123392750.046.2543566
ACACTCG227500.021.72925430-34
CGACCTA153450.021.43293245-49
CGATAGG80550.020.70126535-39
GAAACCG385400.020.4823585-89
CGCGAAA112850.020.02044790-94
AAACCGC387600.019.9585-89
TACGCAC168500.019.36477350-54
GATAAGT415050.018.89080295-99
ATGGGGC2436500.018.88179425-29
TGCGATA83700.018.80386530-34
CTACTTA432950.018.049894135-139
Produced by FastQC (version 0.11.5)