FastQCFastQC Report
Mon 20 Mar 2023
SRR6502980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108653807
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA31820682.9286300110956995No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10415730.9586162038482462No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7466000.6871365307982259No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4427440.4074813503773503No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2655040.24435775177210312No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2480920.22833254245753212No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA2006960.18471142939335755No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1409540.12972762196910415Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC4223700.0141.96461
CGAGTAA6085050.0141.812232
GCGAGTA6100850.0141.795091
TCACGCA3880500.0141.590932
ATGAATC3073150.0141.32932
ATCAGCA4235050.0140.925232
ATAGCGA1480400.0140.884121
TTCACGC3917650.0140.870591
TAGCGAC1471200.0140.772742
TAAGGTC3391200.0140.640352
ATCCGTC2444100.0140.620592
ATCATTC4565600.0140.59141
GTCGTAG2858350.0140.123871
CACGCAT1911200.0139.996863
AGTTAGC1938950.0139.884982
GATAGAC1144100.0139.046361
AGGAGCT1970100.0138.578573
TCGTAGA2889250.0138.12992
CTAAGGT3460650.0137.92391
CAGATTC1856400.0137.636262
Produced by FastQC (version 0.11.5)