FastQCFastQC Report
Mon 20 Mar 2023
SRR6502977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116184678
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20238381.7419147127128072No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT5616910.4834467071467031No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3970490.34173955364407No Hit
CAACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2984490.2568746629396348No Hit
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT2617020.22524656822649197No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2357550.2029140193511575No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2347570.2020550420598489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGAC10048400.0139.066245
CAAGACT2759450.0138.516331
CTAAGTG9787250.0138.20653
TAAGTGA9322950.0137.953084
GCCTAAG11071900.0137.473271
AGTGACT5858950.0137.430286
ACGTATC1984050.0137.258931
ACGCTTA2704100.0137.23752
ACAAGCT1911550.0136.956921
CCTAAGT10528500.0136.955382
GTGACTG5406950.0136.927297
AGAGTCA1435300.0136.788181
AACGTGA2044200.0136.555451
AAGACTA2809800.0136.53772
TGACTGG5464800.0136.44088
CAAGCTA1920800.0136.307792
ACGTGAT2057750.0135.888582
CTCAATG1895800.0135.581041
AGACTAT1313000.0134.892143
AGTGACC1914800.0134.406166
Produced by FastQC (version 0.11.5)