FastQCFastQC Report
Mon 20 Mar 2023
SRR6502974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118987360
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA42488973.570880974248021No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA17541961.4742708805372267No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC13498201.13442301770541No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11986931.0074120478007076No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC9088450.7638164255430157No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3865080.3248311417279953No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2588280.2175256262513934No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT2038630.17133164396621625No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1984500.1667824212588631No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1714050.1440531162301609Illumina Single End PCR Primer 1 (100% over 21bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1525190.12818084206591354No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1214950.1021074843580024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA2209750.0142.553051
GTCTGTC6816650.0142.113851
GATAGAC2143350.0141.667951
ATTGGCG7474350.0141.494261
TAGCGAC2234250.0140.364212
TATCAGC2152850.0140.239651
TCACGCA3223000.0139.856722
TTCACGC3229250.0139.782911
TCTGTCA6953900.0139.717512
ATGAATC2092800.0139.555282
GCGAGTA2145500.0139.375761
CTGTCAT3498200.0138.87463
CTGTCAA2376650.0138.162583
ATAGACA2205350.0138.132572
GTCGTAG1489400.0138.082381
AGATGTA3945800.0137.701071
CGAGTAA2167700.0137.665632
GATGTAC3949550.0137.622962
ATCAGCA2200250.0136.947862
AGTTAGC1021300.0136.849042
Produced by FastQC (version 0.11.5)