FastQCFastQC Report
Mon 20 Mar 2023
SRR7403979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57228374
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAG8134350.0142.844541
ACCGAGA8082000.0142.680862
AACATCG6860000.0142.648212
ACATCGA2230400.0142.39263
ACGCTTA2713850.0142.1992
AACGTGA1918500.0142.130971
CATCAAG2402900.0142.091631
CCGAGAT4080050.0141.947333
GTGCGAA2312200.0141.541262
AAACATC6941700.0141.43081
ACATCGT2546350.0141.403723
ACATCGG2150650.0141.109073
AGTACAA2578350.0140.45261
CCGAGAA2541400.0140.33023
CATCGAT827150.0140.26044
GTACGCA530300.0139.972021
CATCGAA705450.0139.91484
CATCGTA776450.0139.865264
ACAGCAG1686000.0139.480711
GCGAGTA656800.0139.474261
Produced by FastQC (version 0.11.5)