FastQCFastQC Report
Mon 20 Mar 2023
SRR7403978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48247661
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCA6449050.0142.811371
ATACCAA6465700.0142.066912
ATCCTGT6679650.0142.042161
CAACCAC10554000.0141.525991
TCCTGTA6687000.0141.373122
AACCACA10570000.0141.108632
TACCAAG2298900.0140.231553
ACCACAG3551250.0139.968773
GAGCTGA1756900.0139.823331
ATTGAGG6208450.0139.79291
ACCACAT3894050.0139.783513
TTGAGGA6188600.0139.63782
CCTGTAT3444950.0139.454273
AGCTGAA1765050.0139.228352
CTCAATG1726750.0138.994521
TACCAAT2332350.0138.92753
TCAATGA1726000.0138.827222
GTCACTA820700.0138.193972
GATCGCA406400.0137.348422
ACCACAA3300250.0137.262923
Produced by FastQC (version 0.11.5)