FastQCFastQC Report
Mon 20 Mar 2023
SRR7403977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99069859
Sequences flagged as poor quality0
Sequence length150
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2701620.2726984803723199No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1477950.14918260860752816No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1183790.11949042947562892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA5203250.0143.54911
TAGCGAC5201350.0143.22482
GATAGAC2849150.0142.472141
AGCGACT2560200.0141.944933
TATCAGC2100600.0140.494491
AACAGGC8327650.0139.878162
ACCTTAC6047350.0139.866942
TAGGATG5262750.0139.850781
GAACAGG8321500.0139.542191
GTGCGAA1488950.0139.092
ATCAGCA2119750.0138.892682
ATAGACA2934000.0138.863282
AGGATGA5290000.0138.832442
AGTTAGC1788850.0138.80112
GCCACAT2582450.0138.756961
GCGAGTA1732950.0138.72091
ACAGGCT3497300.0138.354263
CACCTTA6116500.0138.34281
GCGACTT1382100.0138.263474
TCACGCA1969200.0138.24462
Produced by FastQC (version 0.11.5)