FastQCFastQC Report
Mon 20 Mar 2023
SRR7403976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53526963
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGC5932500.0143.349671
GATCGCA5936000.0143.138572
ATCGCAT3370850.0142.36353
ACGTATC1222650.0142.241421
CGTGAGA2872100.0142.239792
ATCGCAA1973850.0142.06423
ACCGAGA2819700.0141.48512
GTCACTA1284200.0141.174532
AACCGAG2848850.0141.161621
CGCATAC1063300.0141.106831
ACTTCGA1210850.0141.076452
CCGTGAG2906400.0140.907871
CGAAGTA2129450.0140.814322
AGAGTCA1193550.0140.341161
AACGTGA895000.0140.083081
GAGCTGA1242500.0140.032751
GAGTCAA1193700.0139.991582
CCGAGAT1593200.0139.918643
TCGCATA1350800.0139.905444
ACGTGAT896450.0139.707722
Produced by FastQC (version 0.11.5)