FastQCFastQC Report
Mon 20 Mar 2023
SRR7403972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56170599
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7602421.3534518298442928No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2257050.4018205324817704No Hit
ACCTCTGCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1313320.23380914987216No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1268780.2258797346989303No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT995500.1772279480231286No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC876560.1560531693813698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA1843700.0142.241411
ACGTATC1099250.0141.824681
CATCAAG1246250.0141.380341
AGAGTCA1036050.0141.311261
ATCGCAT1125000.0141.0023
GAGTCAA1033500.0140.776472
TGCCTAA1850100.0140.757192
ATCCTGT2259650.0140.52131
ACGTGAT1302200.0140.517262
CAAGCTA1519050.0140.492362
GATCGCA2056750.0140.49092
AACGTGA1302350.0140.462451
AGTCACT905800.0140.419191
AAGGTAC1368250.0140.242051
GCTCGAT1009900.0139.965363
AGCAGAT888050.0139.894473
ACAAGCT1544500.0139.859731
GTCACTA903800.0139.820712
ACGCTTA801700.0139.801012
AAGACTA1026950.0139.725372
Produced by FastQC (version 0.11.5)