FastQCFastQC Report
Mon 20 Mar 2023
SRR7403972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56170599
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2144568538.179555464594564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA32738235.828356930998724No Hit
AAGCAGTGGTATCAACGCAGAGTACACGTCTGAAAAAAAAAAAAAAAAAA4255400.7575849422577815No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA4034430.7182458566980922No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3893180.6930992493065634No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3155860.5618348488681775No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2691600.47918306870824007No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2564980.45664102674069756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA2233760.3976742352346999No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2232670.39748018353872283No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2034960.3622820543537376No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2008250.3575268976569041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA2007260.3573506488688148No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA1900480.3383407038262134TruSeq Adapter, Index 2 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAAAAAA1847760.32895501078776107No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1832180.32618131773884057No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAGCAGTGGTATCAACGCA1591580.2833475213607745No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1502850.2675510011919225No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1494980.266149912341152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1461750.26023400605003344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1422280.25320719830671556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1322050.23536334373076562No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1295790.23068830011942726No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1220070.21720793826677903No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACATGGGAAGCAGTGGTA1094110.19478339549129609No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1067100.18997483007079916No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1058300.18840817417667205No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1013080.18035769922980527No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG992620.17671522427595976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC985170.17538890763831805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTACATGGGAAGCAGTGG979510.1743812630518681No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA968580.1724354052197307No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTG932620.16603347954327494No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC909540.16192456840276884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC889010.15826963141340186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG885910.15771774126887983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTCAG884220.15741687212557587No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA881770.15698070088232458No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA872940.15540870411583113No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCA857600.1526777380458414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC853350.15192111446060955No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTCTGAAAAAAAAAAAAAAAAAA841210.14975984144302965No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA826840.14720156357955164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAGTACATGGGAAGCAGT823690.1466407719810857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA822820.14648588668246176No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA806440.1435697703704388No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC784820.13972078168509472No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG783190.13943059428652343No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGTAG748430.13324230350472138No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT708070.1260570498812021No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA693730.12350411289009042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATTAACGCAG682260.12146211935535885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTACGCAG675620.12028000627160838No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC659660.11743866217271423No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA646040.11501390611839478No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC621840.11070560240954526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG597400.10635457172176498No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA595010.10592908222324636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAG582040.10362004507019767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACATGGGAAGCAGTGGTAT576590.10264978659031213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG142500.045.298092
GTGACTG147400.043.8411831
AAGCAGT146373900.041.4901471
GACTGGA159300.040.6567153
AGCAGTG150381500.040.3777852
AGTGGTA151336300.040.1121645
GTATCAA152921250.039.7508939
CAGTGGT153303800.039.601124
GCAGTGG153341500.039.5763443
GGTATCA154347400.039.3805248
TGGTATC155057900.039.2017027
GTGGTAT155171550.039.1199076
TGGAGTT187900.034.2160846
ACTGGAG195100.033.1743054
AGTTCAG194600.033.112039
CTGGAGT216750.029.7942715
ACCTAGC258050.022.58288835-39
GCTAAAC270400.021.68995930-34
GGGCTAA289950.021.06081625-29
ACCTTAC283900.020.72022855-59
Produced by FastQC (version 0.11.5)