FastQCFastQC Report
Mon 20 Mar 2023
SRR7403971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92003710
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5059370.5499093460470236No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2131910.23172000346507768No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1995760.2169216871797887No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1278550.1389672220826747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3773250.0142.861241
GCGAGTA8055050.0142.821031
TAGCGAC3794300.0142.416632
ATGAATC4894800.0142.009452
CGAGTAA8108750.0141.751982
GATAGAC1331450.0140.252781
AGATTCG1498800.0140.240073
TAAGGTC3363800.0140.218952
GTCGTAG3429650.0139.92691
TCGTAGA3422000.0139.883512
CAGATTC4226700.0139.560182
AGCGACT1371050.0139.44373
GTACGCA746550.0138.478011
AGAGATC2065950.0138.351782
ATCCGTC1702350.0138.01642
AAGGTCG1182400.0137.560353
ATCAGCA1179550.0137.51862
GCCACAT2925950.0137.342941
TATCAGC1184900.0137.321091
AGTTAGC1382200.0137.244892
Produced by FastQC (version 0.11.5)