FastQCFastQC Report
Mon 20 Mar 2023
SRR7403971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92003710
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG28252843.0708370347239256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA10039761.0912342556620813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC9903551.0764294179006477No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG4513630.4905921728591162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG4490940.4881259679636832No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4284890.4657301319696782No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3669280.39881869981112716No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT3191770.3469175319125718No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3121160.33924284140280864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3052870.3318203146373119No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2979920.3238912865579008No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACACTCGCTTCTGGAACGT2943990.319986009259844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2010850.21856183843021113No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1869130.20315811177614465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1702160.18500993057779952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA1235270.13426306395687737No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC1157640.12582536073817024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1011490.10994013176207784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT966820.10508489277225885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT54025550.0115.052111
AGCAGTG55495250.0111.9667362
GTATCAA55644950.0111.6809169
AGTGGTA55664100.0111.654275
GGTATCA55777500.0111.357438
CAGTGGT56037650.0110.893034
GTGGTAT56093750.0110.801626
TGGTATC56237350.0110.4299557
GCAGTGG57944350.0107.245623
ACACTCG2117050.026.87782930-34
CATGGGG18331750.026.19414125-29
GGTCGGC1806750.026.042435115-119
CGCTTCT2235800.025.99845335-39
CGTCAAG1770650.025.95098195-99
ACGTCAA1739050.025.90085895-99
ATGGGGG6310300.025.8313925-29
TGCCGAC1826250.025.82081280-84
CTGGCGA1774500.025.793531130-134
CTGCCGA1833750.025.52360280-84
AAGGTCG1718850.025.411373115-119
Produced by FastQC (version 0.11.5)