FastQCFastQC Report
Mon 20 Mar 2023
SRR7403969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78231292
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7508650.959801354169122No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6232530.7966799270041456No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4476280.572185360303138No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT3952190.5051929859473624No Hit
AACACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT843370.10780468766897011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA2616100.0141.096651
CGCATAC3328650.0141.095411
AACGTGA2464450.0140.984661
ACGTGAT2460300.0140.953492
GCATACA3343650.0140.44482
ACGTATC1573950.0140.290911
GTCACTA1449700.0139.991962
AACATCG2665100.0139.455892
AGTCACT1459950.0139.292361
CGAACTT2277500.0138.855711
TGCCTAA2655250.0138.752992
GATCGCA1527350.0138.536382
ATCCTGT2552200.0138.198211
GAGCTGA1399900.0138.087431
CGACTGG1945400.0138.074551
GAACTTA2291450.0137.838272
CAAGCTA1225950.0137.827422
AGATCGC1548550.0137.748381
TCCTGTA2560200.0137.444522
GACTGGA1945300.0137.174882
Produced by FastQC (version 0.11.5)