FastQCFastQC Report
Mon 20 Mar 2023
SRR7403968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91578731
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5701130.622538654745063No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3059050.3340349846079435No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2589160.28272503579461045No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1988280.21711154743998362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC4077000.0142.577651
GCGAGTA3619700.0142.242341
ATAGCGA2273950.0142.142531
ATCCGTC7086800.0141.884632
TAGCGAC2286050.0141.6232
ATAGACA4127850.0140.651952
AGAGATC4358450.0140.55822
CGAGTAA3677100.0139.841962
TATCAGC1576350.0138.749851
TCGTAGA2795500.0138.635972
GTGCGAA1322300.0138.223682
ATCAGCA1577000.0138.188832
GCCAAGA4861950.0138.178791
AATCCGT7314200.0137.51731
GGCTAAC2089150.0137.390732
TAAGGTC2220850.0137.38072
GAGATCA1314850.0137.234393
ATGAATC1583800.0137.181842
GTCGTAG2840200.0136.834751
GCCACAT2676100.0136.777081
Produced by FastQC (version 0.11.5)