FastQCFastQC Report
Mon 20 Mar 2023
SRR7403966_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403966_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154023676
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA40533642.6316499549069325No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20559741.3348428328642148No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15511151.007062706385478No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4949510.32134734922181707No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4930500.3201131233876018No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1668590.1083333448034314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTC8811200.0142.66172
AACATCG9046350.0142.269582
TAGGATG5158750.0140.751631
CTAAGGT8949600.0140.5891
ACATCGA3035600.0140.225813
GGCTAAC2897450.0139.824362
ATAGCGA1505650.0139.798051
GTCTGTC5275900.0139.448961
AGGATGA5183900.0139.390882
TCTGTCA5289150.0138.93622
AAGGTCG2263050.0138.813953
TAGCGAC1517300.0138.594022
AGTTAGC1098900.0137.83532
AAGGTCA2977900.0137.77683
GATAGAC1150650.0137.71651
ACATCGT3298800.0137.421423
AGGCTAA2962950.0136.914051
ATGCCTA2594950.0136.534681
CTGTCAT2699350.0136.351173
GAGCTGA1918150.0136.203781
Produced by FastQC (version 0.11.5)