FastQCFastQC Report
Mon 20 Mar 2023
SRR7403966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154023676
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3530157722.919578286133103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27580071.7906383431596582No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA8470000.5499154558549817No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA7496280.4866966037091597No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3669970.23827310809021335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT3355720.21787040065191018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3268320.21219594836835343No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2664820.1730136605751443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2465200.16005331543963408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2337630.1517708225584747No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2325170.15096185602010953No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2161970.14036608241969242No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1912260.12415363985988752No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1847550.11995233771722212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC1805000.11718977542128003No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1784140.11583543818289338No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1774630.11521800064036908No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1759370.11422724386866341No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG1690420.10975065937265385No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1685660.10944161597597502No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1652560.10729259571755707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA1649530.10709587271504933No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA1639850.10646739790835794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1598120.10375807418075128No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1575570.10229401355152698No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1545220.10032353727228273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT238478750.064.964261
AGTGGTA247569650.062.556215
AGCAGTG248003750.062.408572
GTATCAA248487500.062.2326749
GGTATCA252742100.061.121888
CAGTGGT253505400.061.0621534
GCAGTGG255535400.060.5608753
TGGTATC255981750.060.4315767
GTGGTAT256641950.060.3458826
ATGGGGC5262100.021.07385325-29
ATGGGCT6198800.020.7542425-29
ATGGGGG8016500.020.50829725-29
ATGGGGT5657250.020.33303625-29
GGTATAA1103250.020.0444248
TGGTATA1122850.019.7073557
GGGCTAA981450.019.53875225-29
CATGGGC22986600.018.85927825-29
ACCTAGC714450.018.6600135-39
GGGGCTA694500.018.37946125-29
CATGGGG30415900.018.32919525-29
Produced by FastQC (version 0.11.5)