FastQCFastQC Report
Mon 20 Mar 2023
SRR7403965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206099572
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA36581761.7749556510481255No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC16532780.8021743975285888No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10900870.5289127917257392No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA7673090.37230014237972314No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3702830.17966218775068585No Hit
GCTAACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250600.10919964452910169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACG41329650.0143.028261
CTAACGA41352300.0142.946732
GATAGAC11433950.0142.778171
TAACGAT22308600.0142.341573
ATAGACA11612050.0141.089262
TAACGAA14184600.0140.758823
TAACGAG5524000.0140.364243
AACGATT13621300.0139.859364
TAGACAT5760750.0139.850133
AGTTAGC2938650.0139.781922
TAGCGAC2370000.0138.692442
CAGATTC5135750.0138.616822
AACGATA6746350.0138.515214
ATAGCGA2386650.0138.395631
AGCACCT5297750.0138.193911
AACGAAT8548350.0137.433464
CGTGAGA2928100.0137.069152
ACGATTA3942550.0136.390175
ACGATAT4092400.0136.170975
AACGATG2684600.0136.128224
Produced by FastQC (version 0.11.5)