FastQCFastQC Report
Mon 20 Mar 2023
SRR7403964_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403964_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162178149
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20127371.241065465607207No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10978180.6769210320682597No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10353900.6384275602997541No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1855560.11441492034787004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACT14172800.0143.15641
GTCACTA14160900.0142.792022
GATAGAC2878850.0140.45151
GCTAACG2470850.0140.435491
CTAACGA2378200.0139.906812
ACTTCGA2531850.0139.245562
TCACTAT8503650.0138.705753
ACGTATC1889300.0138.078231
TCACTAG1767200.0137.989913
ATAGACA2928700.0137.284592
TATCAGC1775550.0137.072971
AGTTAGC1527550.0137.053622
CAATGGA5415150.0136.74581
TCACTAA4583300.0136.257253
TAACGAT1318450.0136.254363
ATGAATC2071900.0136.235642
AGAGTCA1347000.0136.08981
ATCAGCA1778750.0135.950792
ATGCCTA1646300.0135.673921
CCGTCTA5072650.0135.27812
Produced by FastQC (version 0.11.5)