FastQCFastQC Report
Mon 20 Mar 2023
SRR7403962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97072801
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30711893.1637997135778537No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6365000.6556934521751361No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5472870.5637902629388433No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2789390.2873503155636768No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2492170.25673205824152534No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1155810.11906630777039184No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1069760.11020182677122915No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1045910.10774490786559254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA2813400.0140.17561
AGAGATC3184150.0139.853552
ATAGCGA1065850.0139.725971
CGAGTAA2827550.0139.363342
GATAGAC1106150.0139.063541
ATCCGTC2215400.0138.763872
TAGCGAC1070150.0138.748232
AGTTAGC1423800.0138.470582
GGATTGC2335450.0138.416562
GTCGTAG1484100.0138.070531
GTGCGAA1589300.0138.045472
CGGATTG2335600.0137.964391
GGCTAAC1736950.0137.847182
TCACGCA2182750.0137.451782
GAGATCT1827850.0137.372213
AGCACCT2917800.0137.285631
ATGAATC1096150.0136.724952
ACCTTAC4455250.0136.631882
GAGATCA1022950.0136.396183
TAAGGTC1673400.0135.625562
Produced by FastQC (version 0.11.5)