FastQCFastQC Report
Mon 20 Mar 2023
SRR7403961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90772425
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10140601.1171454326575498No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT6618870.7291718823199886No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4062270.44752247171979814No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1309920.1443081420376287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC1927150.0140.678331
ATGCCTA2270800.0140.486021
CGTGAGA3359550.0140.416412
CAAGCTA2709050.0140.400632
ACGTGAT2067350.0140.285842
GATCGCA2853850.0140.151932
AGATCGC2872900.0139.598021
CCGTGAG3403250.0139.315671
AAGACTA2541650.0139.27962
AACGTGA2084900.0139.04791
GCTGATC2366300.0138.832282
ACTTCGA1329650.0138.608582
TGCCTAA2301000.0138.513472
CGCATAC1956400.0138.508771
ACGCTTA1516700.0138.457212
CAAGACT2563100.0138.302631
ACAAGCT2758150.0138.302441
AGAGTCA1454250.0138.102331
GCATACA1957350.0138.053822
CTGTAGC2012150.0138.028991
Produced by FastQC (version 0.11.5)