FastQCFastQC Report
Mon 20 Mar 2023
SRR7403961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90772425
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1274665714.042433040650836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12353331.3609121933230273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4841270.5333414855888228No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4307630.47455270694817286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2698580.297290724578527No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2373000.26142300373709304No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2060330.22697752098172985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1764590.19439714208362285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA1467720.16169227604087916TruSeq Adapter, Index 2 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1003880.11059305730787737No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA958160.1055562854027531No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGCCACACCTCTGCAGA948530.10449539053297298No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAGCAGCCACACCTCTGC934900.10299383320430185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT86409400.063.1925621
AGCAGTG89053050.061.3680272
AGTGGTA89120650.061.3439985
GTATCAA89250300.061.2688569
GGTATCA90191650.060.6152468
CAGTGGT90888000.060.1420334
GCAGTGG92002300.059.4008453
TGGTATC92062450.059.3848957
GTGGTAT92085300.059.3733676
GAAACCG1069500.023.2589985-89
GATAAGT1078300.023.13229895-99
AAACCGC1068300.022.94409685-89
CATGGGG12234550.022.80822225-29
CTACTTA1141400.022.21859135-139
CGATAGG132100.022.12842635-39
CGACCTA275250.021.80487845-49
CGCAGAT1163900.021.61782390-94
ATGGGGC3700300.021.59769825-29
TACGCAC277850.021.53352750-54
ATGGGGG3840250.021.41507125-29
Produced by FastQC (version 0.11.5)