FastQCFastQC Report
Mon 20 Mar 2023
SRR7403960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230478885
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG45061251.9551140227010384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG31898831.384023963843803No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA28580361.2400424446690639No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13468950.5843897587408061No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13444810.583342374291684No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA12291300.5332939718100423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA11487550.49842092910159647No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC3225540.13994947953692155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA3071970.1332863962787741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA2917690.12659250759565244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2716440.11786068819276004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2529760.10976103082067583No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2385540.10350362463789253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT2355120.1021837640354777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT104396800.0121.212371
GTATCAA107093250.0118.645429
GGTATCA106958450.0118.542748
AGCAGTG106784050.0118.263782
GTGGTAT107917200.0117.621036
AGTGGTA107975300.0117.570055
CAGTGGT107497950.0117.495954
TGGTATC108019150.0117.342337
GCAGTGG108646050.0116.217613
GTGACTG2497900.051.4603581
GACTGGA2529700.050.4892123
TGACTGG2593250.049.5121842
AGTTCAG3131700.041.087119
ACTGGAG3225150.039.8587574
TGGAGTT4235150.030.6932986
CTGGAGT4477100.029.1229365
GGTATAA849750.027.5177638
CATGGGG41298700.026.16561325-29
CGCAGAT5723150.025.69270390-94
AAACCGC5662000.025.56495385-89
Produced by FastQC (version 0.11.5)