FastQCFastQC Report
Mon 20 Mar 2023
SRR6211806_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211806_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99881094
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4078280.40831350926132226No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3419780.34238511644656194No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2324610.23273773913609716No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGAT1074960.10762397135938458No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGAT1039850.10410879159973958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT5575750.0141.788332
AACGTGA5431250.0141.46751
ACGTATC2745850.0139.842091
ATGCCTA2251250.0139.448261
AACATCG4652300.0139.232992
CTCAATG3224150.0139.057371
GTCACTA1843150.0138.265462
AGTCACT1856400.0138.025381
CGCATAC3929900.0137.84361
TGCCTAA2265100.0137.748142
CGAACTT1557150.0137.487951
CGTGATG2044500.0137.323783
CAAGCTA2073400.0137.044622
ACGCTTA1790050.0136.300322
CCGAAGT1533450.0135.79031
ACAAGCT2104900.0135.60991
CAAGACT1941600.0135.328871
CGTGATT1915150.0134.793533
TCAATGA3296050.0134.243182
ACATCGA1574900.0133.723543
Produced by FastQC (version 0.11.5)