FastQCFastQC Report
Mon 20 Mar 2023
SRR6211803_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211803_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104227101
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC7617750.0140.962522
GTACAAG10072500.0140.918992
CTCAATG5469900.0140.68081
AGTACAA10157350.0139.741821
CACCTTA7703650.0139.39071
GACTAGT2953250.0137.574331
TCAATGA5602250.0137.357322
ACAGCAG5646050.0137.205871
GCGAGTA2152100.0136.381361
TAGCGAC1574850.0136.112052
GATAGAC2307900.0135.34411
CCTTACG2314400.0135.239653
ACGCTTA1551950.0135.070422
ACTAGTA3012500.0134.86852
ATAGCGA1591250.0134.709211
CGAGTAA2187100.0134.198872
CAGCAGA5782950.0133.95782
GGCTAAC2078850.0133.505112
TCGTAGA1889650.0133.020112
ATGCCTA1356400.0132.861921
Produced by FastQC (version 0.11.5)