FastQCFastQC Report
Mon 20 Mar 2023
SRR6211801_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211801_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54861871
Sequences flagged as poor quality0
Sequence length151
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCA2117600.0142.489332
TATCAGC2118050.0142.459061
ATGAATC2964350.0142.031272
ACCTTAC3551500.0141.798682
AGTTAGC1531800.0141.393692
CAGATTC1905700.0140.80692
GATAGAC1424050.0140.739351
GGCTAAC1672900.0140.614062
CACCTTA3602750.0139.781561
GCCACAT1680150.0139.757021
TGGAACA3065600.0139.066641
GGATTGC2284500.0139.035632
CCTAATC1861400.0138.903931
CTAATCC1865350.0138.609792
CCTTACG1200500.0138.110983
TCAGCAT748250.0137.980583
ATCATTC1790450.0137.962051
CGGATTG2304550.0137.8261
TCACGCA920900.0137.579512
GCTAACT626200.0137.296423
Produced by FastQC (version 0.11.5)