FastQCFastQC Report
Mon 20 Mar 2023
SRR6211799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33873696
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5409131.5968526138984067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1922190.5674580063539568No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA867220.2560157592487103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA595110.17568499168204144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG532260.15713077191222358No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT501870.14815920884452644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT394620.11649747343779669No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA394020.11632034484810869No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC345430.10197588122654226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT19663200.0124.1316761
AGTGGTA19945100.0121.425065
TGGTATC19893550.0121.3685157
GGTATCA19929100.0121.359738
GTGGTAT19950000.0121.333826
AGCAGTG20040450.0121.2978442
CAGTGGT19971150.0121.2636264
GTATCAA20067050.0120.9441769
GCAGTGG20468550.0118.5067143
GCAGTTG205550.036.8286483
TGGTATA224900.033.4926387
GTTGTAT228250.033.3504076
GGTATAA234450.032.1902058
CAGTTGT249700.030.5201684
TTGTATC254850.030.0401787
ATGGGGT2463550.026.62228425-29
CATGGGG8069200.026.58692625-29
TGTATCA290200.026.3559288
ATGGGGG2273150.026.30904425-29
CGACAAC51950.025.961562
Produced by FastQC (version 0.11.5)