FastQCFastQC Report
Mon 20 Mar 2023
SRR6211798_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211798_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35466500
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC3977050.0143.993271
CGTATCA3995850.0143.31582
GATCGCA2163900.0143.100852
AGATCGC2164100.0143.087621
ACGCTTA1776250.0143.047642
GTCACTA2664200.0142.848882
AGTCACT2671900.0142.437211
CGCTCGA2886850.0142.396272
ATGCCTA1901050.0142.351471
ACGCTCG2892350.0142.125491
TGCCTAA1914650.0141.340332
GTATCAG1389950.0140.713523
ATCGCAT813700.0140.304063
AGCAGGA1979950.0140.252441
GCTCGAT1057950.0139.734563
ATCGCAG782050.0139.474783
GCTCGAA857950.0139.405033
ACCGAGA1163650.0139.171552
CATCAAG882500.0138.903241
AAGGTAC1039050.0138.878451
Produced by FastQC (version 0.11.5)