FastQCFastQC Report
Mon 20 Mar 2023
SRR6211797_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211797_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37819029
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT1856350.0142.983722
AGAGTCA2248450.0142.960451
ATGCCTA1392800.0142.725541
CGACTGG2395250.0142.667451
ACGTATC984000.0142.578411
GAGTCAA2255400.0142.519912
AACGTGA1866400.0142.213791
GACTGGA2403500.0142.177732
CAAGCTA1319250.0141.752732
CGCATAC894500.0141.477511
GTCACTA791500.0141.395312
ACGCTTA787200.0141.292762
AGTCACT793250.0141.083371
CCGAAGT1442050.0141.063571
CAGTTCA1254850.0141.00862
CGAAGTA1443400.0140.931632
GCATACA899600.0140.675452
TGCCTAA1416200.0140.367282
ACAAGCT1335050.0140.075121
CAGATCT886400.0140.030431
Produced by FastQC (version 0.11.5)