FastQCFastQC Report
Mon 20 Mar 2023
SRR6211796_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211796_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32586827
Sequences flagged as poor quality0
Sequence length151
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGA3145400.0144.069762
AACGTGA3993850.0143.931631
ACGTGAT3998000.0143.782232
CACTTCG3166800.0143.09621
AGCAGGA2594800.0142.84321
CGAACTT1619000.0142.32741
GATCGCA962150.0142.151082
GCAGGAA2613400.0141.826552
CTTCGAG1105800.0141.75963
AGATCGC965000.0141.731261
CGTGATG1530500.0141.301763
ATGCCTA986200.0141.17651
CCGAAGT928950.0140.949191
GAACTTA1635550.0140.887192
CGTGATA1199950.0140.593433
CTTCGAA939950.0140.410343
CTTCGAT1165550.0140.127733
CAAGCTA934600.0140.089362
CCGTGAG919400.0139.834821
CAGTTCA1064950.0139.559362
Produced by FastQC (version 0.11.5)