FastQCFastQC Report
Mon 20 Mar 2023
SRR6211793_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211793_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106041655
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20620381.9445547129569036No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8408780.7929695174976286No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8056290.7597288065713421No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA7362300.6942837699015543No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2255250.2126758583690532No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1769860.1669023366336559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1898350.0140.338851
GATAGAC2121850.0139.80341
ACCTTAC7757950.0139.484092
TAGCGAC1910800.0139.045992
GGCTAAC2529500.0138.852722
CCGACAA5599550.0138.51071
AGGCTAA2543250.0138.329731
TATCAGC2364300.0138.225541
ATGAATC1903800.0138.017942
GACAACT2860000.0137.801623
CACCTTA7762600.0137.510961
CGACAAC5723100.0137.425032
AAGGAGC3584000.0136.696182
ATAGACA2208150.0136.175812
ATCAGCA2391350.0136.089112
CGAGTAA1652900.0135.985282
GCGAGTA1653400.0135.65881
CAAGGAG3571900.0135.451031
CCTAATC2943750.0134.997861
AGTTAGC1020200.0134.695832
Produced by FastQC (version 0.11.5)