FastQCFastQC Report
Mon 20 Mar 2023
SRR6211791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97797081
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1227641112.552942147629132No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA14697001.5028055898723602No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3984920.4074681942705427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3966990.40563480621676223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA3201150.3273257204885287No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2240380.22908454701219558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1900390.19431970571800605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1768120.18079476216677676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1752320.17917917202457198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1683850.17217794056654923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1539100.15737688530805946No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCACTATAGGAAAAAATAAA1422520.14545628411956385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC1349390.13797855582213134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1244960.12730032300248306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1222430.1249965732617316No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1148190.11740534464418216No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1133440.11589711966965557No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1125520.11508727954774027No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1097000.11217103708852005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT1070270.10943782667705594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCAAATTGATCCTGTTTG1040210.10636411530524108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT103151200.068.858681
GTATCAA105551800.067.305319
AGCAGTG106266750.066.811352
AGTGGTA106346200.066.764875
GGTATCA106640500.066.578268
CAGTGGT107356100.066.140674
TGGTATC108077700.065.6920557
GTGGTAT108207200.065.6168756
GCAGTGG109001900.065.108613
AGTTCAG685950.025.1563199
ATGGGGG4596550.023.0593425-29
CATGGGG13716750.021.77881825-29
ATGGGGT2262400.021.51853225-29
ATGGGGC2678200.021.36869825-29
ATGGGAC3533900.020.99869225-29
GAAACCG820600.020.64297185-89
TCGTACG114550.020.56179850-54
TGGACGG816550.020.50019570-74
TTAGGGC796400.020.22505645-49
ACACTCG429100.019.89988130-34
Produced by FastQC (version 0.11.5)