FastQCFastQC Report
Mon 20 Mar 2023
SRR6211790_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211790_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85364142
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA7469550.0141.327471
ACGTGAT6134450.0140.757782
AACGTGA6121850.0140.693421
GAATCTG4387200.0140.456821
TGCCTAA7520000.0140.371232
GAGCTGA2731100.0138.609821
AATCTGA4464900.0138.403462
CGAAGTA2476500.0137.993882
GATCGCA1972800.0137.878852
AGCTGAA2747250.0137.86872
GTCACTA2375950.0137.840452
GCCTAAT3399450.0137.739263
TGAGCCA3462100.0137.592642
AGATCGC1979250.0137.476821
ACTAGTA4751700.0137.413212
GACTAGT4745450.0137.000521
ACGCTTA2054250.0136.70752
CGTGATA1954600.0136.597183
GAGCCAT2082300.0136.488683
CTCAATG2642250.0136.461531
Produced by FastQC (version 0.11.5)