FastQCFastQC Report
Mon 20 Mar 2023
SRR6211789_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211789_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144324332
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA61043314.229592415504823No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19218141.3315938992186016No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC17404231.2059110032811375No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12693560.8795162828122427No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6653860.46103521892621685No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA4511530.3125966313150855No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2436620.1688294666764853No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2272650.15746825005225037No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2182580.15122744514071265No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1869690.12954780209895586Illumina Single End PCR Primer 1 (100% over 21bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1797710.12456042408704861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGAGC6972650.0139.963822
CAAGGAG6977900.0139.661151
GGCTAAC2399400.0138.908052
ATGAATC2809550.0138.534422
TGGAACA4747650.0138.210221
ATAGCGA1973000.0137.94951
GTGTGAC4934700.0137.84425
TAGCGAC1974550.0137.723222
TATCAGC3602450.0137.389741
AGTTAGC1860300.0137.388082
TCACGCA3477750.0137.313782
ATCAGCA3605700.0137.085662
AGTGTGA5539300.0137.045724
TCAAGTG6566250.0137.044241
GATAGAC1850250.0136.70441
GATGTAC3876950.0135.964812
GGAACAA4821050.0135.903432
CCGACAA2404350.0135.686571
CGAGTAA2133750.0135.498462
GCCAAGA3500050.0135.49791
Produced by FastQC (version 0.11.5)