FastQCFastQC Report
Mon 20 Mar 2023
SRR6211788_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211788_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123873086
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA46415813.747045585027243No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA14759651.1915138692839218No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC14198541.1462167011807551No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10509160.8483812214059154No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5583320.45072906313159905No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA3422010.276251291584033No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2671970.21570222283797788No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2063550.1665858231706603No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1980660.15989429697424346No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1745910.14094344916861118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAG6844450.0142.037611
AAGTGAC4032250.0139.86515
TGGAACA5483800.0139.7521
CCTAAGT5550900.0139.297912
GATAGAC2289100.0139.220461
ATGAATC2618850.0139.196732
CTAAGTG4714100.0138.592273
AGTTAGC2006450.0138.588552
GGCTAAC2266750.0138.543352
ATAGCGA1743000.0138.291141
GGAACAA5545200.0138.257282
TAAGTGA4425150.0136.979324
ATAGACA2354450.0136.18632
TAGCGAC1768950.0135.878432
GGCTTCA3520100.0135.718032
AGGCTAA2304400.0135.66781
AGCACCT3079550.0135.658391
GTACGCA1856450.0135.592121
TACGCAA1852500.0135.265992
GTGCGAA1532300.0135.259382
Produced by FastQC (version 0.11.5)