FastQCFastQC Report
Mon 20 Mar 2023
SRR6211788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123873086
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4429844535.76115396043334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA35170752.8392567857718505No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA8560500.6910702135894152No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5261270.4247306795924984No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5114320.4128677314134242No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT5080850.4101657724099971No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4843900.39103732347476994No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4183830.3377513336512824No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3868160.3122679933880068No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3623920.2925510388915313No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA3457910.2791494191078763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT3440790.2777673594084836No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3000840.24225116987882261No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2807260.22662388503019937No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2768700.22351102159511874No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2767300.22339800269446747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC2643800.213428121101302No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2616250.2112040705920574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2594010.20940868462742585No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2562860.20689401408793512No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2464800.1989778473751756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2437590.19678124431323202No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2422970.19560100407928804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC2389920.19293295074605635No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2313710.18678068616131838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA2313620.1867734206605622No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1995940.16112781754706587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1995680.1611068283226592No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC1934430.15616225141916623Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1931420.155919260782766No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1929690.15577960171267552No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1928830.15571017581656116No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1853240.14960796245925448No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1842630.1487514406478902No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA1804360.14566198827080162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1767320.14267183107071377No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1744060.14079410276417914No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT1735510.1401038801923446No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1662500.13420994452338098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA1637690.13220708814826815No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1614500.13033501078676604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTAGTATCAACGCAG1607620.12977960361785126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1398070.1128630960239418No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1396490.11273554612177823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1360860.10985921510020344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA1347790.10880410293483767TruSeq Adapter, Index 2 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1334110.10769974681990244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1332350.10755766591622655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA1327060.10713061592733711No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG1272500.10272610791338484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTG1246010.10058762885749048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCACTATAGGAAAAAATAAA1242810.10032929994171615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT280915700.042.692411
AGCAGTG286246250.041.862632
CAGTGGT286924850.041.7673344
AGTGGTA287128200.041.7429735
GCAGTGG289229450.041.410473
TGGTATC290704100.041.2184267
GTGGTAT291346150.041.1376
GTATCAA293078950.040.8935139
GGTATCA293506200.040.8333748
TGGAGTT571950.022.1408846
AACGAAC163100.021.6287145
ATGGGGG4076950.020.7537525-29
ACCTAGC355900.019.69981835-39
ATGGGGC2609700.019.51849225-29
GCATTGA656600.019.40200430-34
CTACTTA707650.018.792906135-139
AAACCGC679300.018.71211685-89
ATACAAC723700.018.356312130-134
ACTACTT723150.018.324392135-139
GATAAGT726650.018.30153595-99
Produced by FastQC (version 0.11.5)