FastQCFastQC Report
Mon 20 Mar 2023
SRR6211787_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211787_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105867299
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA45866944.332493643764351No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12126691.1454613572412005No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11986681.132236310288789No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9835670.9290564785260084No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6134210.5794244358685301No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA2609490.24648687787907012No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2435280.23003137163251894Illumina Single End PCR Primer 1 (100% over 21bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA2130990.2012887851233458No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1919700.18133078090525384No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1411660.133342402548685No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1273290.12027226650979356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAATC2242900.0139.95442
ATAGCGA1639200.0139.640381
TGGAACA3925150.0138.589071
TATCAGC2355600.0138.01171
TAGCGAC1663050.0136.993322
GGCTAAC1428750.0136.550032
TCACGCA2474300.0136.131912
GGAACAA3982450.0135.991462
CGAGTAA1386400.0135.312642
AGTTAGC867950.0134.850052
GGAGAAC4434500.0134.741241
CAGATTC1600850.0134.212422
GATGTAC2378500.0133.971222
ATCAGCA2419300.0133.653122
GATAGAC839200.0133.564881
AGCACCT2005500.0133.501341
TACGCAA929400.0133.00232
GGCTTCA2101100.0132.971542
AGGCTAA1473800.0132.800081
TTCACGC2551600.0132.731871
Produced by FastQC (version 0.11.5)