FastQCFastQC Report
Mon 20 Mar 2023
SRR6211786_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211786_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83397269
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21283922.5521123479475087No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10499951.2590280384361268No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2265400.27163959050025965No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1331600.1596694970910858No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG974770.11688272430119984Illumina Single End PCR Primer 1 (100% over 22bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT939690.11267635154815442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA6058900.0140.638371
AGTCACT1906350.0138.80311
GTCACTA1908200.0138.26532
ATGCCTA1074650.0138.129971
AAGACTA1616750.0137.559372
GAGTCAA1274650.0137.538352
AACATCG1655500.0137.346042
CAAGACT1625900.0137.221941
CTCAATG1768850.0137.204211
AGAGTCA1290300.0137.12411
TGCCTAA1081500.0137.086332
CAAGCTA1335450.0136.983052
GAACTTA1040350.0136.976682
ACGCTTA1250600.0136.871542
CGAACTT1046450.0136.856581
AATGGAA6221350.0136.825712
ACAAGCT1350200.0135.708391
ACGTGAT1308400.0135.543012
ACGTATC1069000.0135.156851
AACGTGA1311400.0134.884841
Produced by FastQC (version 0.11.5)