FastQCFastQC Report
Mon 20 Mar 2023
SRR6211784_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211784_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86305914
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20879722.41926874211656No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7169430.8306997362892189No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2220840.25732187947166635No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1391410.16121838417701018No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1098220.12724736337303605No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATAC1023510.11859094615462853No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG866000.10034074837559799Illumina Single End PCR Primer 1 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA4561500.0139.361681
CAAGCTA2100750.0139.359882
ACTTCGA1640000.0139.316222
ACGTGAT1829300.0138.8242
AACGTGA1835900.0138.545491
ACAAGCT2132500.0137.910641
ACGCTTA1538300.0137.726362
ACTAGTA2171300.0137.156832
AGAGTCA1152100.0136.713521
GAGTCAA1140750.0136.38732
CGAACTT1194200.0136.079041
ACGTATC1123400.0136.007251
AAGACTA1510200.0135.944782
CATCAAG980800.0135.354321
GAACTTA1196500.0135.274892
GCATACA2177400.0135.201542
GACTAGT2191500.0135.094911
AAGCTAG640250.0134.651463
AATGGAA4724650.0134.609392
CTCAATG1488100.0134.475311
Produced by FastQC (version 0.11.5)