FastQCFastQC Report
Mon 20 Mar 2023
SRR6211783_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211783_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130809559
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC27997192.140301535608724No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8653970.6615701532943781No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT3922210.29984123713772326No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2917580.22304027490834977No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATAC1919560.14674462743200595No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1807310.13816345027200957No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1642730.1255818009446848No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1557060.1190325853785655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA11142150.0141.794661
AGAGTCA2450000.0140.279631
CTCAATG4972550.0140.117261
ATGCCTA1863700.0139.847231
GAGTCAA2468250.0139.814212
ATGTGAC2340800.0139.412495
AGTCACT2070650.0139.103991
CGAACTT1970450.0138.56851
TCAATGA4991300.0138.556442
AGCTGAA2476900.0138.38892
GAACTTA1975350.0138.375962
TGCCTAA1896100.0138.27872
AAGACTA2394900.0138.00172
GAGCTGA2487500.0137.538021
GTCACTA2125550.0137.526572
CAAGACT2418200.0136.992831
AGTCAAT1398100.0136.918053
GAATCTG1243800.0136.7781
AATGGAA11606950.0136.356642
ATGGAAT6057550.0135.763983
Produced by FastQC (version 0.11.5)