FastQCFastQC Report
Mon 20 Mar 2023
SRR6211781_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211781_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119722735
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC29571702.4700154068481646No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12193201.0184531785044837No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT3412800.285058639864851No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATAC2001430.16717209141605394No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1426010.1191093738378095No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1255210.10484307763266518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCA3559950.0140.703121
ACACAGA8088200.0140.367071
GTGTGAC6264200.0140.03125
GAGTCAA3567250.0139.866292
ATGCCTA2879000.0139.801911
CACAGAA8161750.0139.394962
CAAGCTA2524150.0139.344532
CAATGGA6512900.0139.083071
TGCCTAA2909500.0138.928512
ACCGAGA1821000.0138.51012
GTGACTG3230850.0137.966377
AACGTGA1843050.0137.96051
TGACTGG3267350.0137.643778
CGAAGTA1827850.0137.612842
ACGTGAT1856150.0137.52812
CCGAAGT1827150.0137.44671
ACAAGCT2543700.0137.086461
GACGGAT1542300.0137.015873
TGTGACT4013450.0136.856
AACCGAG1829150.0136.816131
Produced by FastQC (version 0.11.5)