FastQCFastQC Report
Mon 20 Mar 2023
SRR6211780_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211780_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139217999
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC37531782.6958999748301222No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17115221.2293827035971117No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2995840.21519056598421588No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATAC2643790.18990288748511605No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2156850.15492608825673468No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2129350.15295076895912002No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1883470.1352892595446656No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1716940.12332744417623759No Hit
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1537380.1104296866097034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA2783600.0140.727521
AGTCACT2537800.0140.182511
GCGTGAC4401700.0140.065235
AGAGTCA2104400.0140.050691
ACGTGAT2337950.0139.964872
CTCAATG4420200.0139.80251
TGCCTAA2807000.0139.44372
AACGTGA2354500.0139.437071
CAAGCTA2304850.0139.388372
GAACTTA2823950.0139.363972
CGAACTT2819950.0139.312071
GAGTCAA2096200.0139.310682
CAATGGA5918250.0139.307051
GTCACTA2560800.0138.952322
CGACTGG2082350.0138.239791
GGCGTGA4916050.0138.102744
TCAATGA4493650.0137.758382
ACAAGCT2346100.0137.33211
AAGACTA1979800.0136.958482
AAGGTAC2007200.0136.807221
Produced by FastQC (version 0.11.5)