FastQCFastQC Report
Mon 20 Mar 2023
SRR6211779_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211779_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129224430
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA36586852.831264181238795No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13564281.0496683947454828No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12704740.9831531081235955No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9441350.7306164941102855No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3759360.29091712766695893No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA3386050.26202862724950693No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1607480.12439443532465184No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1350160.10448179187170722No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1310020.10137556807176475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3401300.0141.410451
GGCTAAC3198950.0140.53142
GATAGAC2312600.0140.170961
TAGCGAC3435350.0140.088932
ATGAATC2914150.0139.975022
GCGAGTA3443200.0139.302761
CGAGTAA3455200.0139.200782
TGGAACA4952900.0138.988271
TATCAGC3094150.0138.858571
ATCAGCA3099150.0138.70752
CCGACAA3805100.0138.357181
TCACGCA3715500.0137.781312
AGTTAGC1566500.0137.6342
AGGCTAA3247200.0137.586621
GATGTAC2759950.0137.183152
ATAGACA2386800.0137.115942
CAGTGAC2760600.0136.645455
GGAACAA5037450.0136.401662
CGACAAC3891500.0136.299322
GCTAACT1649450.0136.190643
Produced by FastQC (version 0.11.5)