FastQCFastQC Report
Mon 20 Mar 2023
SRR6211776_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211776_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28180058
Sequences flagged as poor quality0
Sequence length150
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC7020300.0143.685181
ATAGACA7028200.0143.523682
TAGACAT2280350.0142.8883
TAGACAG2384400.0142.525313
GTACGCA727600.0142.136381
GTGCGAA1951900.0142.131442
GGTGCGA1955300.0141.88431
TCACGCA714800.0141.770872
TATCAGC664300.0141.613281
GCCACAT1307100.0141.530181
CTCGGTA828050.0141.51752
TAGACAA1888400.0141.508623
TACGCAA730900.0141.494642
ATCAGCA667250.0140.987182
CAGATTC784100.0140.470112
TGCGAAT684200.0140.125023
ACACGAC1295300.0140.001571
CCTAATC920900.0139.97621
TAGCGAC245300.0139.731062
CCACATA1324050.0139.718352
Produced by FastQC (version 0.11.5)