FastQCFastQC Report
Mon 20 Mar 2023
SRR6211775_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211775_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96402567
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCA5353400.0142.916582
ACGCTTA5811000.0142.50512
AGATCGC5369150.0142.497341
ACGTATC2673450.0142.126271
GAGCTGA5309450.0142.098251
CTCAATG4900600.0141.971761
ACTTCGA3334700.0141.855682
CGCATAC2544600.0141.310731
CGTGAGA3516050.0141.297972
AGCTGAA5342800.0141.211262
ATCGCAT1854700.0140.972213
GCTGATC1965000.0140.859182
CACTTCG3361050.0140.743581
ATCGCAG1789450.0140.681343
CCGTGAG3533100.0140.61611
CGCTGAT1969100.0140.56591
GACGGAT2620100.0140.544243
CAGTTCA4567100.0140.521162
CTGAGCC4016900.0140.432141
AGAGTCA2833900.0140.394781
Produced by FastQC (version 0.11.5)