FastQCFastQC Report
Mon 20 Mar 2023
SRR6211773_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211773_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36530526
Sequences flagged as poor quality0
Sequence length151
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA1549100.0142.428361
CCGAAGT1242900.0141.720731
ACGTATC1024350.0141.567541
TGCCTAA1559000.0141.523912
GAGCTGA1303150.0141.421391
CGAACTT1122200.0141.258961
AGCTGAA1307900.0140.907792
CAGATCT1515400.0140.864381
CTGGCAT1022350.0140.560971
CGAAGTA1253250.0140.550322
AACGTGA1130250.0140.49661
ACGTGAT1130500.0140.465532
ACTTCGA738750.0140.25312
TGAGCCA962100.0139.63542
AGATCTG1529100.0139.60232
GATCGCA673650.0139.601332
ACGCTTA673150.0139.543472
AAGGTAC1159400.0139.465381
CCGTGAG1110500.0139.248081
CGCATAC845400.0139.247441
Produced by FastQC (version 0.11.5)